Surface Modification of Caboxyl-functionalized Polymeric Nanoparticles for Attachment of Targeting Peptides

Download or read book Surface Modification of Caboxyl-functionalized Polymeric Nanoparticles for Attachment of Targeting Peptides written by Amit Kulkarni and published by . This book was released on 2009 with total page 67 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cellular targeting of drug delivery systems represents a promising approach to reduce adverse effects by limiting release of pharmacological agent to desired target site. Controlled surface modification of nanoparticles by specific ligands can facilitate maximum receptor binding and cellular internalization, thereby increasing efficacy of targeting. This research attempts to establish the foundation for ligand-mediated, covalent surface modification of prefabricated carboxyl functionalized polymeric nanoparticles. Control over size and surface charges affecting stability is demonstrated through fabrication of particles in the absence and presence of a stabilizer. Covalent conjugation chemistry for amidation of surface carboxyl groups to amine-bearing ligands is explored and challenges in separating unbound ligands without compromising particle stability are presented. Successful synthesis and purification of a transcytosis-facilitating peptide sequence ( -RYRGDLGRR- ) and its analogues are outlined. Lastly, increased transport of peptide-modified nanoparticles across BeWo cell monolayers, an in vitro model of the human placenta is demonstrated. Preliminary competition studies showing this increased transport of peptide-functionalized particles to be a result of receptor mediated interactions are put forth. In summary, we provide sufficient experimental evidence to suggest increased transport properties of modified particles to be dependent on covalent surface conjugation of appropriate ligands. This establishes the foundation to evaluate controlled expression of targeting ligands for optimizing targeting efficiency of delivery systems.