Matrix-assisted Laser Desorption Ionization (MALDI) Time-of-flight Mass Spectrometry (TOFMS) for Direct Analysis of Bioactive Peptide and Protein

Download or read book Matrix-assisted Laser Desorption Ionization (MALDI) Time-of-flight Mass Spectrometry (TOFMS) for Direct Analysis of Bioactive Peptide and Protein written by and published by . This book was released on 1999 with total page 13 pages. Available in PDF, EPUB and Kindle. Book excerpt: The overall goal of this research was to develop a matrix assisted time-of-flight mass spectrometry (MALDI) method of mixture analysis based on time-lag focusing matrix-assisted MALDI (MALDI-TOFMS). The specific objectives were to develop sample/matrix preparation methods for mixture analysis and to demonstrate the overall analytical performance of the MALDI method for mixture analysis. This report describes and presents results of this research, including direct analysis of peptide and protein mixtures by MALDI-TOFMS. A two-layer sample preparation method is described as very effective for analyzing complex mixtures. In this method, the first layer on the MALDI probe is the densely packed matrix microcrystals formed by fast solvent evaporation of a matrix solution. A mixture solution containing both matrix and sample is then deposited onto the first layer to form uniform analyte/matrix micro-co-crystals. The effect of solvent conditions for preparing the second-layer solution is discussed.