HPLC in Enzymatic Analysis

HPLC in Enzymatic Analysis
Author :
Publisher : John Wiley & Sons
Total Pages : 480
Release :
ISBN-13 : 9780470111000
ISBN-10 : 0470111003
Rating : 4/5 (03 Downloads)

Book Synopsis HPLC in Enzymatic Analysis by : Edward F. Rossomando

Download or read book HPLC in Enzymatic Analysis written by Edward F. Rossomando and published by John Wiley & Sons. This book was released on 2009-09-25 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of High Performance Liquid Chromatography (HPLC) techniques in the study of enzymatic reactions has grown significantly since the publication of the first edition of this highly successful book: the role of enzymes in biological research has expanded; the application of HPLC and enzymes has extended to more disciplines; advances in separation techniques and instrumentation have increased the capability of HPLC; and the discovery of new enzymes has spawned new methods of analysis. High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition addresses these developments in its coverage of the refinements of HPLC methods and their use in a wide range of laboratory applications. It offers the same practical approach found in the first edition, incorporates a wealth of new information into existing chapters, and adds new chapters to deal with new applications, including capillary electrophoresis, forensic chemistry, microdialysis, and the polymerase chain reaction. Topics include: * Application of HPLC to the assay of enzymatic activities * Concepts and principles of HPLC, including the latest technological advances * Concepts and principles of capillary electrophoresis (CE) * Strategy for design of an HPLC/CE system for assay of enzyme activity * Preparation of enzymatic activities from tissues and single cells * Analysis of enzymatic activities in body fluids, including chromatobiosis * HPLC for the identification of new enzymatic activities * Fundamentals of the polymerase chain reaction * HPLC in forensics * Survey of enzymatic activities assayed by the HPLC method, including many new categories * Multienzyme systems, including many new examples * HPLC in the analysis of contaminated food "It is the ability of HPLC to accomplish separations completely and rapidly that led to its original application to problems in the life sciences, particularly those related to purification. An analysis of the literature revealed that this technique was used primarily for the purification of small molecules, macromolecules such as peptides and proteins, and more recently, antibodies. This application to purification has all but dominated the use of the method, and there has been a plethora of books, symposia, and conferences on the use of HPLC for these purposes. However, it was only a matter of time before others began to look beyond and to explore the possibilities that result from the capacity to make separations quickly and efficiently." --from the preface to the First Edition Easy to read and full of practical advice and hundreds of diagrams and examples, High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition is an invaluable resource for students, researchers, and laboratory workers in analytical chemistry and biochemistry, molecular biology and cell biology, and for anyone interested in keeping up with this fast-growing field.


HPLC in Enzymatic Analysis Related Books

HPLC in Enzymatic Analysis
Language: en
Pages: 480
Authors: Edward F. Rossomando
Categories: Science
Type: BOOK - Published: 2009-09-25 - Publisher: John Wiley & Sons

DOWNLOAD EBOOK

The use of High Performance Liquid Chromatography (HPLC) techniques in the study of enzymatic reactions has grown significantly since the publication of the fir
High Performance Liquid Chromatography in Enzymatic Analysis
Language: en
Pages: 280
Authors: Edward F. Rossomando
Categories: Science
Type: BOOK - Published: 1987-05-04 - Publisher: Wiley-Interscience

DOWNLOAD EBOOK

Describes the high performance liquid chromatography (hplc) method and thoroughly explains and illustrates its use in the analysis of enzymatic activities, rath
HPLC in Enzymatic Analysis
Language: en
Pages: 480
Authors: Edward F. Rossomando
Categories: Science
Type: BOOK - Published: 1998-04-21 - Publisher: Wiley-Liss

DOWNLOAD EBOOK

The use of High Performance Liquid Chromatography (HPLC) techniques in the study of enzymatic reactions has grown significantly since the publication of the fir
Methods of Biochemical Analysis, Volume 37
Language: en
Pages: 324
Authors: Clarence H. Suelter
Categories: Science
Type: BOOK - Published: 1993-12-06 - Publisher: Wiley-Interscience

DOWNLOAD EBOOK

Concerned with application of special instrumental methods to problems in biology. Describes the use of x-ray crystallography in biochemistry. Reviews the appli
HPLC of Peptides and Proteins
Language: en
Pages: 411
Authors: Marie-Isabel Aguilar
Categories: Science
Type: BOOK - Published: 2008-02-03 - Publisher: Springer Science & Business Media

DOWNLOAD EBOOK

The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological scien