Implementation of Chemical Protein-nucleic Acid Cross-linking Into Mass Spectrometric Workflows and Mass Spectrometric Database Searches

Download or read book Implementation of Chemical Protein-nucleic Acid Cross-linking Into Mass Spectrometric Workflows and Mass Spectrometric Database Searches written by Fanni Laura Bazsó and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein nucleic acid interactions play a pivotal role in cells, from transcription to translation. Functions of proteins in protein nucleic acid interactions are diverse: histones are responsible for the packaging of the genomic DNA; ribosomal proteins are part of the ribosome, which is responsible for protein synthesis. Crosslinking mass spectrometry proved to be a useful tool to identify protein-nucleic acid interactions and their dynamics in the cell. A vast amount of effort has been spent to elucidate protein-nucleic acid interactions with UV crosslinking mass spectrometry, whereas chem...