Engineering Plasmonic Nanostructures for Multi-dimensional Biosensing with Surface Plasmon Resonance

Download or read book Engineering Plasmonic Nanostructures for Multi-dimensional Biosensing with Surface Plasmon Resonance written by Chih-Yuan Chen and published by . This book was released on 2013 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt: In addition, a new approach for SPR analysis of carbohydrate interactions has been developed with fluorochemistry and calcinated SPR gold film. Fluoroalkysilane was used to provide a monolayer modification of the hydrophobic interface for effective capturing of carbohydrate probes through non-covalent interaction. Molecular recognition with various lectins was investigated by real-time kinetic study. Polydimethylsiloxane (PDMS) channel chips were utilized that enabled parallel analysis for high-throughput detection of carbohydrate-protein interaction with SPR imaging technique. Matrix-free LDI-MS of the calcinated gold film and array is not compromised by the SAM coating, allowing for the development of new SPR-MS on-chip analysis. Finally, a novel label-free biosensing approach based on thin-film transmission interferometry (TTi) has been developed with nanoscale porous anodic alumina (PAA) film. The optical phenomenon of TTi has been successfully confirmed by simulation. Performance of TTi sensing in relation to the structural geometries of PAA nanofilm was studied, providing valuable insights into the optimization of TTi-substrate based on porosity, thickness, and pore diameter to achieve high biosensing sensitivity. This newly developed substrate also provides a convenient platform for biological studies of protein adsorption. As a surface-sensitive label-free detection, TTi shows a great potential to be incorporated into the ongoing on-chip SPR-MS biosensor development for achieving higher level of research possibilities.